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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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Low molecular weight hydrophobic protein
Posted by: k_ocall (IP Hidden, New member, 1)
Date: July 25, 2005 02:56PM
I'm working on anovel protein that has a low molecular weight. When i run lysate on a 15% SDS-PAGE gel and probe with an antibody generated to a peptide sequence in the novel protein i get a very diffuse/smeared band (sometimes it even looks like two bands very close to each other). Anyone have any ideas about whats happening? I haven't looked at any post-translational modification because i don't really know what to look for.
Re: Low molecular weight hydrophobic protein
Posted by: mito lab (IP Hidden, Unregistered user, )
Date: July 25, 2005 05:10PM
The hydrophobic protein tends to run as a fuzzy band and the migration pattern sometimes changes in different type of polyacrylamide gel, probably because of its different charge conditions. I would suggest to try a grediant 10-20% tris-tricine gel. Good luck.
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