Proteomics and protein biochemistry forum / Carbamylation trains

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Carbamylation trains

Posted by: monsa (IP Hidden, New member, 9)

Date: June 13, 2005 01:23PM

I got spots across the gel in my IEF. I suspect these are "carbamilation trains". I found on the web that they can be prevented by using a mix of urea and thiourea. What is the concentration range for both in the mix? Is there any other way to prevent this problem?
Thanks,
Monsa

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