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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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Student needs help
Posted by: MatrixCore (IP Hidden, Unregistered user, )
Date: April 28, 2005 03:05PM
Could anybody please tell me what are the activators of the enzyme citrate synthase?
I can't find it anywhere Tnx for your answers!
Re: Student needs help
Posted by: femmeauburn (IP Hidden, Advanced member, 115)
Date: April 28, 2005 03:56PM
Citrate synthase is a mitochondrial enzyme involved in the citric acid cycle. It catalyzes the reaction of 2 carbon acetyl CoA with 4 carbon oxaloacetate to form 6 carbon citrate. Coenzyme A is regenerated.
Re: Student needs help
Posted by: MatrixCore (IP Hidden, Unregistered user, )
Date: April 29, 2005 06:27AM
Yes, thank you for that, I know that, just need to know what are/is the ACTIVATORS for the citrate synthase.
I know which inhibitors are used. But the activators are a mystery
Re: Student needs help
Posted by: femmeauburn (IP Hidden, Advanced member, 115)
Date: April 29, 2005 09:11AM
Ah now I understand what you were asking. Here is the answer: NADH is a potent inhibitor of citrate synthase (as you already know), most of the adenylates are activators. The best activator, ADP-ribose, increases the affinity of the enzyme for the substrate, acetyl-CoA, and saturates the enzyme in a sigmoid manner. The paper below should tell you more.
Talgoy MM, Duckworth HW. Can J Biochem. 1979 May;57(5):385-95. The interactions of adenylates with allosteric citrate synthase.
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