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Position: Discussion forums > Bioscience biotechniques discussion forums > Proteomics and protein biochemistry forum
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S-35 pulse labeled membrane protein runs slower?
Posted by: mitolab (IP Hidden, Senior member, 89)
Date: June 9, 2005 09:10AM
hi, just a quick and maybe naive question. If a 40kD protein is in vivo pulse labeled with S-35 and resolved on 10% - 20% Tricine gradient gel, will it run slower than unlabeled protein (noticeable difference)? Assume there are 300 aa with 4% methanione. I am trying to use a labeled protein as the marker to identify the unlabeled counterpart.
Re: S-35 pulse labeled membrane protein runs slower?
Posted by: 5'-ATCG (IP Hidden, Unregistered user, )
Date: June 10, 2005 02:23PM
No difference between labeled and unlabeled proteins.
Re: S-35 pulse labeled membrane protein runs slower?
Posted by: femmeauburn (IP Hidden, Advanced member, 115)
Date: June 14, 2005 12:34PM
The label is generally not heavy enough to change the run speed.
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