The alkaline (pH>13) SCG comet assay

A detailed protocol by Vasquez and Tice (Integrated Laboratory Systems).

PROTOCOL FOR THE APPLICATION OF THE pH>13 ALKALINE SINGLE CELL GEL (SCG) ASSAY TO THE DETECTION OF DNA DAMAGE IN MAMMALIAN CELLS

The general principle of comet assay: The comet assay works because DNA strand breaks create fragments or supercoiled DNA-loops that, when embedded in an agarose gel, migrate in an electric field.

Last update 08-Apr-2008, Rating Good of 1 votes.