Amplification of Orientation-specific RNA

This protocol describes the procedure for amplifying RNA from a starting sample of total
RNA to produce increased amounts of RNA having the sense orientation. This protocol
is specifically designed for amplifying RNA that will be used with spotted oligonucleotide arrays or mixed cDNA/oligonucleotide spotted arrays, because it provides RNA in the appropriate (sense) orientation for interaction with both cDNA and oligonucleotide elements on the array. (Jeremy Hasseman from Institute for Genomic Research). (TIGR)

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