Technique / Molecular Biology / Cloning library construction and screening

Gene construction advice needed


Gene construction advice needed (archive)

I'm looking for advice on a gene construction project. I'm trying to
assemble a 219 bp gene from oligonucleotides. I designed the oligos so
that each overlaps the next by ten complementary bases. The plan then is
to fill the gaps, then ligate. I can get perfect results with any two
adjacent oligos, but when ... ...

Last update 26-Jun-2006, Rating n/a of 1 votes.

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By surekha agarwal on 26-Jun-2006
i want to prepare the hairpin construct with conserved potion of coat protein of papaya ringspot virus, i want strategy to prepare that
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