General Tips To Make Cloning Easy!

A general tips and guidance on subcloning (EZ clone system) from Flemington lab at Tulane University.

Whenever possible, we strongly suggest the use of directional cloning strategies (i.e. cloning into a
vector cut with two different restriction enzymes) over non-directional cloning approaches (cloning
into single enzyme digested + phosphatase treated vector).

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Last update 23-Apr-2005, Rating n/a of 0 votes.