Technique / Cell Biology / Cell culture / Cell preservation

Trypan blue staining of cell culture

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By Mangala Kulkarni on 07-Aug-2007 Hi i a, scraping J77A.1 cell line, when check the vabilty with trypan blue dilution 1:1 and 1:10 i am getting more (more then 50%) dead cells in 1:1 dilution when compared with 1:10 dilution, i am following this protocol, cell suspension (20 microliter) and 20 microliter of trypan blue loaded on heamocytometer, 1:10 dilution, 10 microliter cell suspension+90 microliter of trypan blue i didnt find a single dead cell, i am noting getting which method is correct, please suggest me, thanks and regard Mangala Kulkarni Rating: Good Reply