Dialysis of FBS (fetal bovine serum)
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Dialysis of FBS (fetal bovine serum)
PDF protocol from Marimoto lab at Northwestern University.
Reference:
Current Protocols in Protein Science, Desalting, Concentration, and Buffer Exchange by Dialysis and
Ultrafiltration, Unit 4.4, 2001 John Wiley & Sons, Inc.
Last update 27-Mar-2007, Rating Very Good of 1 votes.
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After FBS dialysis, how to compare the final concentration of the exchanged protein/chemical? We are doing FBS dialysis in order to get rid of possible antibiotics in the FBS, but how do we know if the trace of antibiotics is dialyzed out?
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