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Preparation of E.Coli competent cells with CaCl2 method

Preparation of E.Coli competent cells with CaCl2 method

Preparation of E.Coli competent cells with CaCl2 method protocol from Marimoto lab at NothWestern University. (PDF file).

Last update 19-Apr-2007, Rating Good of 2 votes.

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By rojalin Nayak on 11-Jul-2008

hi, i've prepared competent cells of E.Coli (DH5 alpha) by a glycerol n distilled water tratment only n after that it was growing in LB+ampicillin plate.i dont find the reason kindly suggest me
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By Berenice Serafin on 19-Apr-2007

Necesito conocer el protocolo para transformar celulas competentes de E. coli
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