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Long PCR of circular mitochondria DNA



Long PCR of circular mitochondria DNA

I need a protocol for PCR of circular DNA. I would like to amplifie the whole
mtDNA (16-17kb) of mytilus by using a long PCR method for circular NA. ... ...

Last update 26-Jul-2002, Rating Good of 0 votes.


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By jaikumar on 26-Jul-2002
I have designed primers recently for the first time,but correctly by following all the rules for primer design.Only positive control of plasmid isn't amplifying,but other transformed plants and negative control plants also amplifying .What could be the problem?
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