Protein extraction from whole tissues for isoelectric focusing
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Protein extraction from whole tissues for isoelectric focusing
Protocol from University of Missouri - Columbia Proteomics Center.
1. SDS extraction followed by acetone precipitation.
2. Phenol extraction followed by methanolic ammonium acetate precipitation.
Last update 25-Apr-2005, Rating Very Good of 9 votes.
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je veux bien comment , on fait l'extraction des proteines membranaires des cellules eucaryotes Rating: Very Good
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i have a problem of protein disoving ,so i have problem of band separation in 2D .what is percursor to increase the solubility in extracted protein sample? Rating: Excellent!
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I want to know how to extract protein from rice, corn, soybean... to determinate protein by spectrophometer. Rating: Excellent!
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I want to know a method for protein extraction from brain tissue for use in performing ELISA. Rating: Good
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I need to know about extraction of pure malarial proteins from red blood cell without red blood ceel proteins contamination please guide me. Rating: Good
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How to purify protein 10?
The steps please. Rating: Very Good
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I want to know how to extract protein from rice, corn, soybean... to determinate protein by spectrophometer. Rating: Good
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the protocol is a good one for the IEF gels, but use of phenol to precipitate protein s in DNA extrction protocols is a good method because it is really useful in theextraction of protein plant tissue samples with poor protein concentrtion . Rating: Very Good
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Protein purification tips Rating: Very Good
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