Fluorescence Resonance Energy Transfer (FRET)

FRET is an elegant experimental tool to examine the spatial relationship between two proteins in vivo. (University of Washington, Dept. Biochemistry)

This page is the first in a series of linked web pages that describe the approach. The pages include:

Introduction to FRET
Microscope set up
Preparation of the yeast
Mounting cells
Image acquisition
Image analysis-Spillover factors
Determine FRETR values

Last update 04-Feb-2007, Rating Very Good of 2 votes.