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Calcium Phosphate Transfection of PC12 Cells

Calcium Phosphate Transfection of PC12 Cells

Calcium Phosphate Transfection protocol for PC12 cell line. (Finkbeiner lab, UCSF)

PC12 cell transfection protocol describes in detail the procedure, notes, media and reagent preparation etc.

Last update 27-Mar-2008, Rating Poor of 1 votes.

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By Qian Liang on 27-Mar-2008

I transfected pc12 cell line with the methods of Calcium Phosphate-DNA co-precipitation and Lipofectamine.But both of the methods' transfection efficiency are below 5%.Could you give me some advice or protocols about pc12 transfection?
I look forward to hearing from you soon.
Thank you.
Rating: Poor

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