SDS-PAGE gel electrophoresis problems
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SDS-PAGE gel electrophoresis problems
The "Hall of Shame" presents examples of some of the worst gels students have run in past labs. They represent many of the ways one can mess up a gel (but not all of them - we're still finding new ways!). See what features of your own gel(s) were unsatisfactory - or at least less than perfect - and use the illustrations to figure out what you might do to improve your technique.
Last update 26-Nov-2006, Rating Very Good of 4 votes.
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it is really a nice site regarding the troubleshooting of SDS-PAGE but still some aspects of troubleshooting are missed here, i do hope that they will also be added soon.
like there is big band of protein at the top and then narrowing of the band till the end. i have not seen that in this site.
hope such type of problem will be discussed.
Rating: Good
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Bonjour,
je trouve un problème pour le coloration pourtant j'applique exactement ce qui se trouve dans le protocol. Je charge les puits avec marqueurs de protéines et les échantillons chacun dans un puit bien sûre et pas de problème pour la migration. une fois la migration est finie je fait la coloration dans le mélange d'acide acétique + isopropanol + eau et bleu de bromophénol selon le protocol pendant 20 à 30 min puis dans la solution de décoloration (acide acétique + isopropanol+ eau) mais rien n'apparait.
Rating: Very Good
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this is greatful & appreciate efforts. you give the troubleshooting gel photos that is very remarkable.
i also doing with SDS PAGE and face with certain problems related to it which are not showing in your troubleshooting picture.
so i want tell about that things plz give me your e mail address or other contact sources.
Rating: Very Good
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this is the first time i am surfing this site i find itvery fruitful.
i am very excited by watching the various gel at the 'Hall of Shame".
this is very nice of troubleshooting the gel problem.
i really appreciate the person who are working behind this work.
Rating: Very Good
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