Immunostaining Protocol with Video Demonstration
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Immunostaining Protocol with Video Demonstration
A step by step immunostaining protocol with video demonstration on how to perform immunocytochemistry fluorescent staining on cell cultures on coverslips.
You are recommended to first download their detailed protocol in pdf format including buffer and reagent for immunostaining. Quicktime movie video is also available for download for important steps of the immunostaining technique. ( Angus Lamond's research laboratory, University of Dundee, Scotland)
Last update 18-Jan-2002, Rating Good of 2 votes.
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please I want some protocol for immunohistochemistry for heat shock protein (Hsp 60 Hsp70)
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i want some protocol of immunohistochemisry for rats brains
Rating: Excellent!
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hai, your streptavidin bead conc seems ok.how long is the spacer arm in your biotin . keep it as long as possible so that there would be minimum steric hinderance in later binding. i think this is critical. i use biotin- 21- dUTP which has 21-atom spacer arm, for 3'-end labelling and incorporate it through PCR. it worked fine in my clone capture procedure.
hope it will help
harsha
Rating: Good
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hai, your streptavidin bead conc seems ok.how long is the spacer arm in your biotin . keep it as long as possible so that there would be minimum steric hinderance in later binding. i think this is critical. i use biotin- 21- dUTP which has 21-atom spacer arm, for 3'-end labelling and incorporate it through PCR. it worked fine in my clone capture procedure.
hope it will help
harsha
Rating: Good
Reply
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GeBAflex-tube recover 80%+ oligo of DNA extraction.
Visit www.geba.org
constact: Muller. M at muller@geba.org for further detail
Rating: Good
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Be sure that the salt buffer is 2M other wise the biotin will not bind
good luck.
Rating: Good
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