A Concise Guide to cDNA Microarray Analysis

Efficient expression analysis using microarrays requires the development and successful implementation of a variety of laboratory protocols and strategies for fluorescence intensity normalization. The process of expression analysis can be broadly divided into three stages:
I. Array Fabrication
II. Probe Preparation and Hybridization
III. Data Collection, Normalization and Analysis

The procedures described in this article have been tested and refined over the past year and have been optimized using hybridization of RNA derived from cell lines to give reproducible and consistent results. (Priti Hegde, Rong Qi, Kristie Abernathy, Cheryl Gay, Sonia Dharap, Renee Gaspard, Julie Earle-Hughes, Erik Snesrud, Norman Lee, and John Quackenbush, The Institute for Genomic Research, Rockville)

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