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Immunocytochemistry: Common problems and some answers

Immunocytochemistry: Common problems and some answers

Results obtained from immunocytochemical labeling can often differ from what was expected. Sometimes this may result in the formulation of new theories but often good results are ignored because of faulty interpretations. (House Ear Institute)

Last update 16-Mar-2005, Rating Fair of 3 votes.

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By Hongyan Yin on 01-Jun-2007

I made an new antibody. it recognize overexpressed protein very well. but for endogenouse protein, the background is too high. any advise to optimize the fix or wash condition
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By Sanghamitra P Chatterjee on 25-Oct-2005

I am interested in immunostaining a protein and to localize its position in the cell. please advise.
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By varsha on 16-Mar-2005

will you please send me the detailed procedure of immunocytochemistry of nuclear proteins in human spermatozoa.
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