Cell cycle methods (method booklet)

Method booklet on cell cycle analysis (BioSource international).

Content:

Introduction ................................................................................2
I. Cell Proliferation ....................................................................4
Detection of Proliferating Antigens ..............................4
Measuring DNA Synthesis ............................................5
alamar Blue™ Reduction ..............................................5
II. Cell Synchronization ........................................................13
Serum Starvation ........................................................13
Chemical Inhibitors ....................................................13
Propidium Iodide Staining ..........................................15
Intracellular Antigen Detection ..................................17
FITC conjugated................................................................18
Unconjugated ..................................................................19
III. Immunological Methods................................................22
IP ................................................................................25
Western Blot ..............................................................29
IHC ............................................................................40
IV. Regulation of Protein Phosphorylation: Case
Studies ....................................................................................46
Case Study 1: Regulation of Cyclin B1/cdk ................46
Case Study 2: Regulation of Retinoblastoma ..............49

Last update 01-Mar-2005, Rating Very Good of 6 votes.