G1/S cell synchnronization using double thymidine block

This protocol is designed to synchronize HeLa cells at the G1/S border (actually all G1). It is highly effect and gives almost complete synchronization in the culture. Note that this protocol will not work on cell lines with intact p53 apoptotic responses. (Science Advisory Board)

Last update 25-Oct-2006, Rating Excellent! of 1 votes.