Large-scale Immunocytology

This protocol describes our method for preparing cells for immunofluorescence, in which all incubations and washes are performed in microtiter dishes. Two dishes can be processed simultaneously. The last step is to transfer each sample to a well on a polylysine coated 8-well slide for visualization. The protocol can also easily be adapted for preparing cells for immunofluorescence in microfuge tubes.

Protocol from Yale Genome Analysis Center YGAC.

Last update 31-Jan-2005, Rating n/a of 0 votes.