Interpreting Electrospray Mass Spectra

An IonSource.Com tutorial on interpreting electrospray mass spectra.

This tutorial mainly focuses on the interpretation of mass spectra containing multiply charged molecules generated by the electrospray ionization process. In this tutorial we use peptides to demonstrate the complexity of ESI spectra. While small molecule pharmaceutical drugs (mw 200-500) in most instances ionize quite well in electrospray they routinely display only a single charge state [M+H]1+ making interpretation fairly straight forward. Don't be discouraged, if your business is low molecular weight analysis the majority of the principles discussed in this tutorial still apply in full scan mass analysis of small molecules.

Last update 16-Dec-2005, Rating Good of 1 votes.