Home Forums /Molecular /Cell /Genetics /Proteomics /Neuroscience /Immunology /Bioinformatics /Histology /Pharmacology /Jobs /Books /Bioproducts /Blog /Methods

Technique / Proteomics and protein biochemistry / protein gel staining and protein digestion for mass spec

Silver Staining (and De-Staining) of Polyacrylamide Gels

Silver Staining (and De-Staining) of Polyacrylamide Gels

A very detailed procedure on Silver Staining (and De-Staining) of Polyacrylamide Gels including buffer preparation. (Molecular structure facility, UC Davis).

Last update 21-Feb-2007, Rating Poor of 1 votes.

Post your message in protein gel staining and protein digestion for mass spec forum

Write your comment

By sajida on 21-Feb-2007

we recently start to work on PAGE.Our PAGE gels Sometimes stain completely and I get a "chrome-plated" surface with no trace of DNA bands in silver staining. So please help me and reply me soon.

Rating: Poor

Reply

Related resource

Coomassie Blue: destaining

Precautions on gel preparation and staining

What if silver stain does not work?

Silver staining of SDS-PAGEs

silver staining protocol from Breeden lab

Reduction/Alkylation and Proteolysis of Electrophoretically Separated Proteins

Trypsin In-gel Digestion Procedure

Sypro Ruby Staining Protocol

About the site \| Terms of service \| Privacy policy \| Ad Sponsorship \| Contact us Copyright 2000-2008 Biowww.net, All rights reserved