Suspension Culture Infections: Low MOI

Infection of suspension cultures is the most common way of producing protein in the baculovirus system. Suspension cultures are easily scaleable from about 10 mL to more that 100 L. Generally, the suspension cultures are maintained in serum free media. These media are available from several commercial sources, all of which work well, though there is variation in effectiveness dependent upon cell line and protein. (Dr.Jim Litts, baculovirus.com)

Last update 28-Jan-2005, Rating n/a of 0 votes.