biowww.net
Home Forums /Molecular /Cell /Genetics /Proteomics /Neuroscience /Immunology /Bioinformatics /Histology /Pharmacology /Jobs /Books /Bioproducts /Blog /Methods
Search biowww:

Technique / Proteomics and protein biochemistry / Protein expression, protein extraction and protein purification / Baculovirus expression system


Freezing and recovering Sf9 Cells



Freezing and recovering Sf9 Cells

Sf9 cells can be frozen in a manner quite similar to methods used to preserve mammalian cells. This involves putting them in a suitable medium, including 7.5-10% DMSO, and a controlled freezing rate. (Dr. Jim Litts, baculovirus.com)

Last update 28-Jan-2005, Rating n/a of 0 votes.


Write your comment


Your Name
Your Email
Your Comment
Your Rating
Related resource
INSECT CELL CULTURE & BACULOVIRUS INFECTIONS


Sf9 Spinner Culture Media Recipe


Baculovirus Protein Expression Protocol


Plaque assay for baculovirus culture


Suspension Culture Infections: Low MOI


Suspension Culture Infections: High MOI


Recovering Virus Stocks from Frozen Cell Pellets


Recovering Low-Titer Virus Stocks