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Degenerate PCR
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Degenerate PCR
The identification of novel members of gene families by PCR using degenerate primers has been considered more of an art than a science, so much so that the methods books I've come across have been too timid to discuss the considerations that go into the design of this experiment, much less give a protocol for its execution. (by Michael Koelle, Yale University)
Last update 14-Aug-2005, Rating Excellent! of 2 votes.
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Now, after making many combinations, iam able to get some good results using degenerate primers. iam also trying mixtures of gene specific and degenerate primers.
Rating: Excellent!
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yes, i agree with the statement that it is more of an art than a science.
i have tried many different primer combinations, but not many good results.
Rating: Very Good
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