Technique / Molecular Biology / PCR / Degenerate PCR
Degenerate PCR
The identification of novel members of gene families by PCR using degenerate primers has been considered more of an art than a science, so much so that the methods books I've come across have been too timid to discuss the considerations that go into the design of this experiment, much less give a protocol for its execution. (by Michael Koelle, Yale University)
Last update 14-Aug-2005, Rating Excellent! of 2 votes.
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By N.Senthil kumar
on 04-Dec-2005
Now, after making many combinations, iam able to get some good results using degenerate primers. iam also trying mixtures of gene specific and degenerate primers. |
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By N.senthil kumar
on 14-Aug-2005
yes, i agree with the statement that it is more of an art than a science. |
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