Tryptic digestion directly in polyacrylamide gel slices

This protocol is designed for the direct digestion of proteins in Coomassie stained polyacrylamide gels. Direct digestion in the gel band eliminates transfer of the protein of interest to a membrane and allows Coomassie staining to be used. (Biomolecular Research Facility, University Virginia Health System)

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Last update 26-Jan-2005, Rating n/a of 0 votes.