PCR primer design and reaction optimization

From Molecular Biology Techniques Manual edited by Vernon E Coyne et al. (Written by
Ed Rybicki, University of Cape Town)

Contents

* Factors Affecting the PCR

* Denaturing Temperature and Time
* Annealing Temperature and Primer Design

* Primer Length
* Degenerate Primers
* Elongation Temperature and Time
* Reaction Buffer
* Cycle Number

* Nested Primer PCR

* Labelling of PCR products with digoxygenin-11-dUTP
* Helix Destabilisers / Additives
* Useful Universal cDNA PCR Primer
* A simple set of rules for primer sequence design
* REFERENCES

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Last update 12-Apr-2005, Rating Excellent! of 1 votes.