Cell fractionation based on density gradient
|
Cell fractionation based on density gradient
A general notes on techniques of cell fractionation. In the study of cell organelles, different fractions of the subcellular particles are routinely separated with a centrifuge. It was by this type of isolation technique that mitochondria were discovered to be responsible for the entire tricarboxylic acid cycle and ribosomes accountable for protein biosynthesis. (Dr.Nam Sun Wang, University of Maryland)
Last update 03-Jun-2005, Rating Good of 5 votes.
|
Write your comment
|
I need to separate nuclei from avian erythrocytes,
Rating: n/a
Reply
|
|
|
using a known fractionation technique(iso-density centrifugation).How can the inner and outer membrane of a mitochondrion be seperated for a close research.
Rating: Excellent!
Reply
|
|
|
We want to isolate DNA from organelles without nuclear DNA contamination? Is there any compamy or institution offering such service:
Mitochondria from animals.
Mitochondria and chloroplasts from plants
Thanks.
Rating: Good
Reply
|
|
|
I need to isolate a protein that migrates from cytoplasm into nucleus. How can I separate cytoplasmic proteins from nuclear fractions for western and immunoprecitation analysis?
Rating: n/a
Reply
|
|
|
how can i seprate intact cells from te homogenate,i need to isolate broken cell walls from the homogenate
Rating: Excellent!
Reply
|
|
Related resource
Mitochondria isolation from tissue or cell protocol
Mitochondria: Architecture dictates function
Protocols For Mitochondrial Research
Golgi apparatus isolation protocol
Blue Native Gel Electrophoresis for Thylakoid Protein Complexes
Subcellular Fractionation Protocol
Mitochondrial DNA: Methods and Protocols (Methods in Molecular Biology, Vol 197)
Mitochondrion and chloroplast databases
|
