In situ PCR: Amplification and Detection in a Cellular Context

A general introduction and protocol on performing in situ PCR. (Ernest F. Retzel, Katherine A. Staskus, Janet E. Embretson and Ashley T. Haase, University of Minnesota)

Table of Contents:

Introduction
Technical Aspects/Philosophy
Primer Design
Reaction Conditions
"Mechanics" of the Reaction
Methodology
A. Cell and Tissues.
1. Cultured Cells: Infection and Preparation.
2. Tissues: Pulmonary infection and preparation.
B. In Situ Amplification
1. Cultured Cells in Suspension.
2. Tissues on Slides.
C. Detection of Amplified DNA by in situ Hybridization
1. Solution amplification.
2. Tissue Sections.
References

Last update 25-Jan-2005, Rating n/a of 0 votes.