Home Forums /Molecular /Cell /Genetics /Proteomics /Neuroscience /Immunology /Bioinformatics /Histology /Pharmacology /Jobs /Books /Bioproducts /Blog /Methods

Technique / Proteomics and protein biochemistry / Immunoprecipitation IP

co-immunoprecipitation question: starting concentration

co-immunoprecipitation question: starting concentration

What is a good starting concentration of each protein to have? What volume? I know that my proteins are tight-binding, so dissociation should not be a big problem. (post from NWFSC forum).

Last update 15-Jun-2007, Rating n/a of 1 votes.

Post your message in Immunoprecipitation IP forum

Write your comment

By Alvaro Morales on 15-Jun-2007

Hi! I need to do a Co-IP and my buffer have a 420mM NaCl. I would like to know if this concentration is to higth and may disociate the protein-protein interactions
Thanks
Rating: n/a

Reply

Related resource

Immunoprecipitation TroubleShooting Guide

Immunoprecipitation western blot troubleshooting

CO-IMMUNOPRECIPITATION ANALYSIS OF PROTEIN-PROTEIN INTERACTIONS

Phosphatase inhibitors cocktails selection guide

How to remove RNase from immunoprecipitation?

Immuno-precipitation of Phospho-Tyr proteins

troubleshooting tips of IP, western and immunostaining

Anti-FLAG Co-Immunoprecipitation

About the site \| Terms of service \| Privacy policy \| Ad Sponsorship \| Contact us Copyright 2000-2008 Biowww.net, All rights reserved