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Immunoprecipitation TroubleShooting Guide

Immunoprecipitation TroubleShooting Guide

Troubleshooting guide from Sigma. It addresses immunoprecipitation problems on:

1. No binding
2. High background or unwanted precipitates

Last update 19-Oct-2007, Rating Excellent! of 1 votes.

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By Knowledge on 19-Oct-2007

Can some tell me how best to troubleshoot my Co-IP experiments. I am pulling down proteins that are showing 2 distinct bands at 55 kDa and 43 kDa on comassie. But i dont seem to be getting my protein of interest.
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