Troubleshooting Tips for Immunoprecipitation / Kinase Assays

A brief troubleshooting guide on immunoprecipitation - kinase assay. (Upstate)

Determine the optimal stimulation time and temperature for each individual kinase that is to be tested. This is essential since optimal stimulation periods are often short and can easily be missed if stimulation occurs for too short or long a period of time. As an example, optimal stimulation time of Akt is two to five minutes at 37?C; after five minutes, activity of the kinase drops dramatically ... ...

Last update 22-Jan-2005, Rating n/a of 0 votes.