Some vector sequence and map

This is collection of some useful cloning vector sequences and maps (by Dana Boyd at the Dept. of Microbiology and Molecular Genetics, Harvard Medical School).

Arabinose Promoter Plasmids

pBADphoA - Generates phoA fusions under arabinose promoter control

pBAD18

pBAD22

pBAD24


Cassettes to generate phoA Fusions


Signal sequenceless phoA and its flanking linker sequences of pSWFII and pSWFIII


Plasmids expressing TEV protease in E. coli


pGEX2T-27k

pMM13


Transposons to generate TEV protease inserts, phoA or uidA fusions


TnTIN

TnTAP

TnphoA


Plasmids expressing trehalases from E. coli


ptre11

pBADtreF


Plasmids expressing degP and degPSA from E. coli


pCS10 (degPSA in pACYC184 - cmR)

pCS20 (wt degP with C-terminal 6 His tag)

pCS21 (degPSA with C-terminal 6 His tag)


Cloning Vector Classics



pBR322

pBR325

pBR327

pBR328

pBR329

pACYC184

pACYC177

pHSG575

pGEX-2T


6-His tag plasmids


pCS19 (introduces C-terminal 6 His tag)

pNTH1 (introduces N-terminal 6 His tag)


Bacteriophages of E. coli


186

fd

lambda

phi-X174

T7


M13 phage cloning vectors


M13mp8

M13mp18

M13tg130

M13tg131

Last update 10-Mar-2005, Rating Excellent! of 3 votes.