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Troubleshooting GST fusion protein expression in E. coli



Troubleshooting GST fusion protein expression in E. coli

By M. Saluta and P. A. Bell, Amersham Pharmacia Biotech.

The production of soluble, intact GST fusion proteins requires optimization of several factors. This discussion focuses on choosing the proper host strain, growth temperature, cell density at time of induction and length of induction for expression of a GST-luciferase fusion protein. Examples demonstrate that for this fusion protein, E. coli BL21 grown at 28°C gave superior results compared with other strains and growth conditions.

Last update 15-Apr-2008, Rating n/a of 1 votes.


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By Victor on 15-Apr-2008
I am trying to express a GST-fusion protein in BL21, I seem to be getting all of my protein in the pellet and not in the lysate. I am planning to solubilize the protein which could be in the pellet by adding Lysis buffer containing 8M Urea. Is this advisable.
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