DNA Sequencing: Troubleshooting your data

Troubleshooting guide on DNA sequencing from Roswell Park Cancer Center.

As mentioned in other sections of this website, the two most common causes for failure to get good or any sequence data for your samples are purity and concentration of your template DNA. If you are having trouble getting good sequencing results for your samples, you may first want to look through our Sequencing Basics section for some recommendations on template preparation and quantitation. If it appears that you have done everything correctly and followed our suggestions, then look below for some additional reasons why you might obtain less than optimal DNA sequence data quality.

Last update 20-Jan-2005, Rating n/a of 0 votes.