Ceramide ELISA

I have coated ELISA plates with Ceramide - 1000 - 100 - 10 and 0 ng/ml (100
mcl/well) using 2 coating buffres (ph 9.6 and 7.2).


Hereafter we added a mouse anti ceramide antibody and a biotinylated rabbit
anti mouse antibody, TMB etc., with no result. All wells gave the same as
the "0" = bagground.

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Last update 04-Sep-2003, Rating Good of 0 votes.