Real-Time PCR Method
A background introduction on real-time PCR technique (Davidson college, Dept. of Biology).
Real-time PCR is able to detect sequence-specific PCR products as they accumulate in "real-time" during the PCR amplification process. As the PCR product of interest is produced, real-time PCR can detect their accumulation and quantify the number of substrates present in the initial PCR mixture before amplification began.
Last update 11-Jul-2006, Rating Good of 2 votes.