Home /Forums /Molecular /Cell /Genetics /Proteomics /Neuroscience /Immunology /Bioinformatics /Histology /Pharmacology /Jobs /Books /Journals /Blog /Methods /Buffer

Technique / Immunology / ELISA assay

ELISA question

ELISA question

We have been having a problem with developing an ELISA. Here is the method
we are using:

1) We coat the plate with a goat polyclonal The antibody is in a 50mM
carbonate/bicarbonate buffer, pH=9.5, and the coating is for 2 hrs @ 37C
or overnight @ 4C. We are using EIA/RIA plates from CoStar.

2) After 4 washes in PBS, ... ...

Last update 23-Jan-2002, Rating Good of 0 votes.

Post your message in ELISA assay forum

Write your comment

By Joseph Peterson on 06-Sep-2002

We are non profit and would like to open a lab in Ethiopia. Price list for new and old lab equipment is my interest. Thanks JP
Rating: Good

Reply

By shiyongqi on 30-May-2002

ÎÒ¡ã´ÕÕ¡¶ÖÐ¹¨²É¨²ÎïÖÆÆ¡¤¼¨¬¶¡§¹æ³Ì¡¡¤ÓÃ¼ÐÐÄ¡¤¡§¡Áö¡¡ã¾¨²Ìåµ¡ã¡ã¡Á²ÐÁôÁ¿²â¶¡§¡¡À¡£µ«½¨¢¹û¡ÁÜÃ»ÓÐÌÝ¶È£¬ÎÒÊÔÓÃµ¡ÁÎïÒº¼ÓÁ¨°Ë¨¢ÖÕÖ¹Òº¹Û²¨¬ÑÕÉ«Óë½¨¢¹ûÑÕÉ«´¨®ÖÂÏ¨¤Í¬£¬ÎÒÏë¿ÉÄÜÊÇµÚÒ»²½¿¹ÌåÃ»ÓÐ¡ã¨¹¡À»ÉÏ¡£Çë´¨®¼Ò¡ãïÃ¦½â´ðÒ»ÏÂ¡££¡§Ë¨´ÓÃÊÔ¼Á¼¡ã¿¹Ìå¾¨´Óë¹æ³ÌÉÏÒ»ÖÂ£¬Ò¨°ÎÒÊÇÐÂÊÖ£¬¿ÉÄÜºÃ¶¨¤µØ¡¤½´æÔÚÎÊÌâ£¬Â¨¦¡¤³´¨®¼ÒÏ¨ºÏ¸Ö¸µ¼£¬ÌØ¡ÀðÊÇÈçºÎ¡ã¨¹¡À»£¬ÈçºÎÏ´µÓ£©¡£¡¤Ç³£¸ÐÐ»¡£
Rating: Good

Reply

By oasis on 23-Jan-2002

hrp
Rating: Good

Reply

Related resource

Cytokine ELISA Protocol

Double antibody sandwich ELISA (DAS-ELISA)

Ceramide ELISA

Edge Effect in MicroWell ELISA

ELISA protocol

ELISA assay introduction

Troubleshooting on establishing ELISA

Animation on sanwhich ELISA

About the site \| Terms of service \| Privacy policy \| Ad Sponsorship \| Contact us Copyright 2000-2008 Biowww.net, All rights reserved