Protocol for Transfecting siRNA into Mammalian Cells Using Lipofectamine 2000

Invitrogen manual on Transfecting siRNA into Mammalian Cells Using Lipofectamine 2000.

Two general guidelines for siRNA transfection in mammalian cells are: 1. transfect cells with siRNA when cells reach 30-50% confluent. 2. Don't use antibiotics in transfection medium to avoid cell death.

Last update 12-Jan-2005, Rating n/a of 0 votes.