Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells

Written by Louise Cramer (actin part)and Arshad Desai (tubulin part), Mitchinson lab at Harvard medical school.

Optimal conditions for fluorescence of proteins of the actin and tubulin cytoskeleton are based on: preserving cell structure; properties of individual cytoskeletal proteins and any antibodies to be used; background fluorescence.

Last update 11-Jan-2005, Rating n/a of 0 votes.