Resolving marginally different proteins on SDS-PAGE gel electrophoresis

We occasionally need to resolve hyper- and under-phosphorylated forms of a
protein (pRb) on SDS-PAGE gels for Western blot analysis. Individuals
familiar with these isoforms will know that they differ by an apparent
(maximum of) 6 Kd, and separation is tricky. I'm wondering what those of
you with an interest in the theoretical aspects of SDS-PAGE think about
the use of higher versus ... ...

Last update 26-Apr-2006, Rating Excellent! of 1 votes.