Yeast Two-Hybrid Review
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Yeast Two-Hybrid Review
A full accessible online review from Biological Procedures Online (Wim Van Criekinge and Rudi Beyaert, Biol. Proced. Online 1999;2:1-38)
Abstract:
Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financially and labour intensive procedure, more refined functional studies can be undertaken. Indeed, by making hybrids of two-hybrid systems, customised approaches can be developed in order to attack specific function-related problems. For example, one could set-up a "differential" screen by combining a forward and a reverse approach in a three-hybrid set-up. Another very interesting project is the use of peptide libraries in two-hybrid approaches. This could enable the identification of peptides with very high specificity comparable to "real" antibodies. With the technology available, the only limitation is imagination.
Last update 15-Dec-2005, Rating n/a of 1 votes.
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On -his/-leu/-trp i.e. QDO the putative clones after Cotransformation in (AH109 comp.cells) have grown, but on plate supplemented with X-alpha-gal they are not showing blue colour (marker for third reporter MEL 1 ) and after plasmid isolation and amplification by AD/LD PCR using primer sets showed no amplification.So what may be the possible reason.
Note:- In AH109 strain, both HIS and ADE reporter genes are under same promoter while the MEL 1 is under a different promoter.
thanks.
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