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The Gietz Lab Yeast Transformation Home Page

The Gietz Lab Yeast Transformation Home Page

This site contains information on TRAFO yeast two hyb technique. (Gietz lab at University of Manitoba, Canada)

Last update 04-Oct-2006, Rating Fair of 2 votes.

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By Jia-Ching Shieh on 04-Oct-2006

Why use PEG for transformation and single stranded DNA as a carrier
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By PEG on 04-Oct-2006

Polyethylene glycol(PEG) have two effects on Ligation reaction for further transformation.
1.Increase the rate of ligation of blunt-ended DNA, allow ligations react at low concentration of enzyme and DNA. Ligation by PEG is highly depend on magnesium ions concentrations.

2.Alter the distribution of ligation products and suppress intramolecular ligation, keep all of DNA products are linear multimers.

Reference:Molecular Cloning( Sambrook and Russell
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