MTT cell viability assay
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MTT cell viability assay
The MTT assay, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide], has been used to test cytotoxicity of reagents and cell viability.
MTT cell proliferation assay (introduction and protocol from ATCC)
MTT cell viability assay (protocol from Daniel Ge)
Recent MTT assay technique papers:
1: Wang X, Ge J, Wang K, Qian J, Zou Y.
Evaluation of MTT assay for measurement of emodin-induced cytotoxicity.
Assay Drug Dev Technol. 2006 Apr;4(2):203-7.
2: Burton JD.
The MTT assay to evaluate chemosensitivity.
Methods Mol Med. 2005;110:69-78.
3: Zhang X, Le Pennec G, Steffen R, Muller WE, Zhang W.
Application of a MTT assay for screening nutritional factors in growth media of
primary sponge cell culture.
Biotechnol Prog. 2004 Jan-Feb;20(1):151-5.
4: Plumb JA.
Cell sensitivity assays: the MTT assay.
Methods Mol Med. 2004;88:165-9.
5: Molinari BL, Tasat DR, Palmieri MA, O'Connor SE, Cabrini RL.
Cell-based quantitative evaluation of the MTT assay.
Anal Quant Cytol Histol. 2003 Oct;25(5):254-62.
Last update 22-Mar-2001, Rating Fair of 14 votes.
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Sir,
I'm undergraduate pharmacy student currently doing a project on 'Investihation into the neurotoxic effects of Echis carinatus and Daboia russelli snake venoms on selected central nervous system tissues'. We chose to work on the normal cells and tumor cells-lymphoma.
We need to use MTT assay to test the cell viability and Annexin V to test epitose induction and have no idea what are the procedures for these two, the appropriate concentrations of substances to use within the two tests for a size of 30people or more and if any necessary dilution with set conditions.
Would be grateful if you can point me at the right direction.
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dear sir,i am doing work in drde gwalior,i am doing on cell line plz suggest me that what is the concentration of mtt,if i am taking 1ml media.
Rating: Fair
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dear sir, i am student from Malaysia doing my master project ,
i wonder whether MTT assay is applicable on microalgae cells?
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Why are you still using MTT, while you have AquaBluer or alamarble etc that is 10x cheaper, simpler, nontoxic, and more reproducible assay available?
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SIR
IAM STUDENT IN MEDICAL SCHOOL OF AMERICAN UNIVERSITY AND I HAVE ARESEARCH ABOUT THE HUBBLE BUBBLE ( WATER PIPE) AND THEIR EFFECT ON THE LUNG CELL AND IN MY PROCEDURE I HAVE MANY ERRORS TO COMPLETE THIS RESEARCH AND AS YOU KNOW THE RESEARCH NEED THE MTT AND NO AND OTHER FACTORS OF CELL CULTURE AND IF YOU HAVE THE CORRECT PROCEDURE OR CORRECT KNOLEDMENT ABOUT IT PLEASE HELP ME NOW !
THANKS
Rating: Excellent!
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Respected sir,
i am Anil taneja doing my M. pharm project work on nanoparticle of anticancer drug i want to perform MTT assay for my project, so it will be very kind on your part if you please tell me about MTT And Price to perform
Thanks
Rating: Good
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what is the accuracy and efficiency of MTT assay?
Rating: Fair
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Sir,
my self an research scholar from india and want to perform MTT assay of the compounds synthesised be me. hence intrested to know the prize and avalibility of chemicals/ kit required. I am also intrested to know whether any lab can do these test for me at an economic rate for me.
thanking you
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Dear sir,
This is neelesh kumar doing the M.Pharm (pharmaceutics) from Dept. of Pharmaceutical Sciences Sagar (M.P),India. working with group on drug delivery via the various nanoparticles as carriers and want to do MTT assay so tell me thy price of MTT in Rs. thank sir
Rating: Good
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sir, i wish to work on MTT assay.......so can u plz tell me the approximate price of the reagent MTT in Rupees.........Thanx...........
Rating: Good
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Sir
I want to know how to decide the volume of MTT to be added in medium
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hello, its better if you sentrifuge your 96-welles plate then remove the media slowly.
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What happens with overincubation with MTT reagent, in case formazan crystals are not found within 5 hours incubation.
Rating: Fair
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what is MTT mean
Rating: Excellent!
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I am working with suspension cells and would like to know if the cell washing step, done before addition of MTT can be eliminated without affecting the overall quality of the assay.
Rating: Good
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hello, Im a 10th grade biology student looking for a substance that regulates or is regulated by The Cell Cycle please help I have gone through countless research attempting to find it in my teachers text books from her colledge years thank you so much,p.s. she said it was one sertain substance!
Rating: Good
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Have you tried searching in "Current Immunology protocols"?
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Related resource
Technical guide to Cell Proliferation and Apoptosis Methods (Roche)
MTT Cell Proliferation Assay
Radioactive Proliferation Assay
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