Technique / Cytometry and fluorescence microscopy / Fluorescent confocal microscopy

Immunofluorescence Double Staining Method Parallel Approach

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	By flora gupta on 29-Nov-2007 my quiery is ,how to do double antibody staning (FITC and TRITIC) of two proteins in same(one) cell. suggest me the protocol to do this in confocal microscopy. Rating: Good Reply

	By Patrícia Veras on 24-May-2007 Would you please have any protocol to double staining cells with monoclonal antibodies produced both in the same animal specie (both are anti-rat). One of the antibodies we can biotinylate and reveal it with streptoavidin-FITC. The other one can we use a second antibody? Will it work? Rating: n/a Reply