Estrogen Receptor Binding protocols

From NIH publication No.03-4504
appendix:

Appendix A - Methods for In Vitro ER Binding Assays
A1 - Assays Using Rat Uterine Cytosol
A2 - Assays Using Mouse Uterine Cytosol
A3 - Assays Using Human ERa and ERb
A4 - Assays Using Recombinant ERa and ERb
A5 - Assays Using Fluorescence Polarization
A6 - Assays Using GST-ERdef Constructs
A7 - Assays Using MCF-7 Cells and Cytosol
Appendix B - In Vitro ER Binding Assay Protocols
B1 - Protocol for the Competitive ER binding MCF-7 (Whole Cell Assay)
B2 - Protocol for the Fluorescence Polarization Assay of the Competitive Binding of Ligands to Estrogen-Receptor Complexes
B3 - Protocol for the Competitive Ligand Binding Assay
B4 - Standard Operating Procedure for the Rat Estrogen Receptor Equilibrium Exchange Assay
B5 - Protocol for the Estrogen Receptor Competitive Binding Assay Using Rat Uterine Cytosol
Appendix C - Chemical and Product Class Information for the Substances Tested in the In Vitro ER Binding Assays
Appendix D - Substances Tested in the In Vitro ER Binding Assays
D1 - Data Sorted by Substance Name and Assay
D2 - References
Appendix E - Assay Distribution of Substances Tested in In Vitro ER Binding Assays
Appendix F - Median/Single RBA Values for Substances Tested in Two or More In Vitro ER Binding Assays

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