EMSA protocol

Protocol from Jonathan Flint.

The EMSA, or Electrophoretic Mobility Shift Assay, is a method for studying DNA-protein interactions in vitro. DNA-protein complexes will migrate more slowly than unbound radioactive probe and are thus visualised by discrete bands of radioactivity near the top of an acrylamide gel image.

Last update 06-Mar-2006, Rating Poor of 1 votes.