Index of Typical Cell Culture Contaminants
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Index of Typical Cell Culture Contaminants
This is a trouble-shooting guide for cell culture contamination. If you have a contamination problem, select what you believe to be the organism from the short descriptions in this page and click on the image to go to a more detailed page.
This troubleshooting guid is from UNC Lineberger Comprehensive Cancer Center core facility.
Last update 21-Mar-2005, Rating Fair of 4 votes.
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am facing the problem of contamination eventhough taking a lot of precautions, it may be fungal contamination (rhizobium) in in-vitro culture of frog oocytes. kindly tell me to overcome this matter.
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kindly inform me to overcome the problem of fungal contamination in in-vitro culture of frog oocytes. am using or2 medium in which i've added gentamycin, also maintaining sterile conditions using ethnol but still getting contamination.
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Hi
Would you tell me how I can identify teh contamination and perhaps its source and since I got to realise it is contamination in my stem cells after a lengthy transfection process do you think there is a way to clean them (doubt it) but prevent it (I did use sterile conditions ethanol etc.)
Must say that once when passaging the air flow was turned off and didn't realise till later but I filtered the opened media (0.2 micron filter).
also I didnt see any contamination after spliting etc and only got the bacteria after transfection...
Thanks in advance
Nanaz
Rating: Good
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spirochete
Rating: Very Good
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Related resource
Understanding and Managing Cell Culture Contamination
Fungi contamination in cell culture work
Mycoplasma Detection and Elimination
Detection of Mycoplasma by Culture Protocol
Mycoplasma Detection Using DNA Staining
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